Transfection Reagents
Eurofins Discoverx PathHunter β-Arrestin-2 Retroparticles, 4 vials x 0.5. (93-1087)
The PathHunter β-Arrestin-2 Retroparticles are MMLV particles designed to transduce cells of interest with β-Arrestin-2 fused to EA. In conjunction with a plasmid expressing a ProLink-tagged GPCR of interest, this allows for these cells to be used for β-Arrestin-2 recruitment assays. This product uses EFC technology. Ligand induced recruitment of β-Arrestin-2 to the activated GPCR leads to complementation and produces EFC signal. These retroparticles ship as 4 vials of cryopreserved retroparticles. For detailed product information, User Manual and datasheet, visit www.discoverx.com/products/virus/arrestin/β-Arrestin-2-retroparticles-93-1087.
HORIZON DISCOVERY BIOSCIENCES LIMITED DHARMAFECT4 TRANSFECTION,.75ML
DharmaFECT 4 Transfection Reagent0.75ml
Lonza Walkersville Cell Line Nucleofector Kit C
The Cell Line Nucleofector Kit C is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device. This kit has been determined to be optimal for transfecting following cell types:A2058CCRF-CEMMDA-MB-453MEG-01MG-63NCI-H1299 [H1299]NS0 (ECACC)SK-BR-3T2U266B1U-937The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution C0.5 mL Supplement 230 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Sigma Aldrich Fine Chemicals Biosciences REAGENT KIT FOR MRP2 MRP3 MRP5
NC3883695 REAGENT KIT FOR MRP2 MRP3 MRP5
Lonza Walkersville Cell Line Nucleofector Kit L
The Cell Line Nucleofector Kit L is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device.This kit has been determined to be optimal for transfecting following cell types:3T3-L1 adA-10BHK-21BxPC-3DU145EL4FDC-P1GH3 H9c2 (2-1)HeLa S3IMR-32KG-1aL-428 (DSMZ)MDCKMDCK II (ECACC)MOLT-4MV-4-11NTERA-2 cl.D1WEHI-231The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette format
Lonza Walkersville SF Cell Line 4D-Nucleofector LV Kit XL, Catalog Number V4LC-2002, Reaction Volume Up to 20 mL, Kit Size 5 reactions, Processing Time Less than 10 min, Compatible System 4D-Nucleofector LV Unit
The Lonza SF Cell Line 4D-Nucleofector LV Kit XL is specifically designed for efficient, large-scale transfection of hard-to-transfect cell lines using the 4D-Nucleofector LV Unit. This closed-system nucleofection kit modifies up to 1 x 10^9 cells effectively, retaining cell viability. It allows variable reaction volumes and rapid processing, making it ideal for transient protein production and generating modified cell lines for various assays.
- Compatible system: 4D-Nucleofector LV Unit
- Reaction volume: Up to 20 mL
- Cell numbers supported: Up to 1 x 10^9 cells
- Processing time: Less than 10 minutes
- Kit size: 5 reactions
- Optimal cell lines: HEK293 suspension clones, CHO-S suspension clones, K-562, U-937
- Applications: Large scale transient protein production, generation of modified cell lines
- Key benefits: High transfection efficiencies, maintained cell functionality
- Storage conditions: 2 to 8 °C